Aquatic green alga toxicity testing method based on in-situ actual water body

The invention discloses an aquatic green algae toxicity test method based on an in-situ actual water body. The method comprises the following steps: S1, sampling the in-situ actual water body; s2, in-situ actual water body pretreatment; s3, preparing green alga concentrated mother liquor; s4, toxicity experiment: adding an optimized culture medium formula into the pre-treated in-situ actual water body, dividing the in-situ actual water body added with the culture medium into a plurality of series, putting each series into an experimental group triangular flask, adding an exposed pollutant mother solution into each experimental group triangular flask, uniformly shaking, placing in darkness for balancing, and performing toxicity experiment on the exposed pollutant mother solution; measuring the actual concentration of the exposed pollutants; and inoculating green algae concentrated mother liquor for exposure culture, taking algae liquid every 24 hours in the exposure culture process, measuring the biomass, and