Exogenous insertion sequence of corn transformation event LD02 and application thereof

The invention relates to an exogenous insertion sequence of a corn transformation event LD02, a detection method and application. A nucleic acid molecule of the corn LD02 comprises a sequence as shown in SEQ ID NO: 1 or a reverse complementary sequence of the sequence as shown in SEQ ID NO: 1, or a...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Hauptverfasser: LI WENLAN, YUE RUNQING, LI WENCAI, LU SHOUPING, ZHAO SUXIAN, ZENG TINGRU, ZHAO MENG, QI SHIJUN, ZHANG QINGWEI, SUN QI
Format: Patent
Sprache:chi ; eng
Schlagworte:
Online-Zugang:Volltext bestellen
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:The invention relates to an exogenous insertion sequence of a corn transformation event LD02, a detection method and application. A nucleic acid molecule of the corn LD02 comprises a sequence as shown in SEQ ID NO: 1 or a reverse complementary sequence of the sequence as shown in SEQ ID NO: 1, or a sequence as shown in SEQ ID NO: 2 or a reverse complementary sequence of the sequence as shown in SEQ ID NO: 2. The corn transformation event LD02 disclosed by the invention has insect-resistant and glufosinate-ammonium herbicide-resistant characteristics and excellent agronomic traits, and the detection method can accurately and quickly identify whether a biological sample contains DNA molecules of the transgenic corn event LD02 or not. 本发明涉及玉米转化事件LD02的外源插入序列、检测方法和应用,所述玉米LD02的核酸分子包括SEQ ID NO:1所示序列或其反向互补序列、或者SEQ ID NO:2所示序列或其反向互补序列。本发明玉米转化事件LD02具有抗虫和耐草铵膦除草剂特性且农艺性状优良,检测方法可以准确快速地鉴定生物样品中是否包含转基因玉米事件LD02的DNA分子。