Lysophosphatidylcholine acyltransferase, nucleic acid molecule and application thereof
The invention discloses lysophosphatidylcholine acyltransferase, a nucleic acid molecule and application of the lysophosphatidylcholine acyltransferase, and belongs to the technical field of microbial synthetic biology or metabolic engineering. According to the invention, lysophosphatidylcholine acy...
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| creator | HAO XIAHUI ZHENG MINGMING GONG YANGMIN YOU LINGJIE WAN XIA |
| description | The invention discloses lysophosphatidylcholine acyltransferase, a nucleic acid molecule and application of the lysophosphatidylcholine acyltransferase, and belongs to the technical field of microbial synthetic biology or metabolic engineering. According to the invention, lysophosphatidylcholine acyltransferase (PtLPCAT) from phaeodactylum tricornutum is successfully applied, through overexpression in recombinant cells, the transfer efficiency of fatty acyl between a PC library and a CoA library can be greatly improved, substrate sources for synthesis of long-chain polyunsaturated fatty acids are increased, and the yield of the long-chain polyunsaturated fatty acids is increased. The relative content of the long-chain polyunsaturated fatty acid in the recombinant yarrowia lipolytica is effectively improved. According to the lysophosphatidylcholine acyltransferase provided by the invention, the loss of intermediate metabolites can be reduced, and the relative content of long-chain polyunsaturated fatty acids, |
| format | Patent |
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According to the invention, lysophosphatidylcholine acyltransferase (PtLPCAT) from phaeodactylum tricornutum is successfully applied, through overexpression in recombinant cells, the transfer efficiency of fatty acyl between a PC library and a CoA library can be greatly improved, substrate sources for synthesis of long-chain polyunsaturated fatty acids are increased, and the yield of the long-chain polyunsaturated fatty acids is increased. The relative content of the long-chain polyunsaturated fatty acid in the recombinant yarrowia lipolytica is effectively improved. 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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Lysophosphatidylcholine acyltransferase, nucleic acid molecule and application thereof |
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