Porous scaffold with cell adhesion layer as well as preparation method and application of porous scaffold

The invention provides a porous scaffold with a cell adhesion layer as well as a preparation method and application of the porous scaffold. According to the preparation method, reactive groups in a hydrogel precursor and a cell adhesion layer material are designed, so that the cell adhesion layer is successfully modified on the surface (including the inner walls of holes) of porous hydrogel, and the porous hydrogel scaffold with the cell adhesion characteristic is formed. The porous scaffold with the cell adhesion layer has the functions of cell amplification and sphere culture and recovery, and has a wide prospect in the fields of cell culture and tissue engineering; when the porous scaffold is applied to cell culture, cells can be adhered to the inner walls of the holes of the porous scaffold during cell inoculation, after the cells proliferate to a set number, the cell adhesion layer is split to enable the cells to form three-dimensional cell microspheres in the holes of the porous scaffold, and then the s