Fermentation process for producing IAA (indoleacetic acid) through strain combination co-culture

The invention discloses a fermentation process for producing IAA (Indoleacetic Acid) through strain combination co-culture, which comprises the following steps: activating 5-49 parts of bacillus safensis, 5-54 parts of bacillus isothermal layer and 6-30 parts of bacillus halotolerant, inoculating a...

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Bibliographische Detailangaben
Hauptverfasser:	ZHAO HANFANG, LIANG JIAXING, DING YANQIN, QIAO MINHANG, SUN LONGHAO, CHENG QI, LIU KAI, SUN SHANSHAN
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	AGRICULTURE ANIMAL HUSBANDRY BEER BIOCHEMISTRY BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES, AS HERBICIDES CHEMISTRY COMPOSITIONS THEREOF CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPSOR SEAWEED CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE FISHING FORESTRY HORTICULTURE HUMAN NECESSITIES HUNTING METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PEST REPELLANTS OR ATTRACTANTS PLANT GROWTH REGULATORS PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTSTHEREOF PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TRAPPING VINEGAR WATERING WINE
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Beschreibung
Zusammenfassung:	The invention discloses a fermentation process for producing IAA (Indoleacetic Acid) through strain combination co-culture, which comprises the following steps: activating 5-49 parts of bacillus safensis, 5-54 parts of bacillus isothermal layer and 6-30 parts of bacillus halotolerant, inoculating a single colony into an LB liquid culture medium to prepare a seed solution, and fermenting for 2-3 hours to obtain the IAA. The preparation method comprises the following steps: inoculating seed solutions of 5-49 parts of bacillus safensis, 5-54 parts of bacillus isothermal layer and 6-30 parts of bacillus halotolerant in a volume ratio of 1: (0.8-1.2): (1.8-2.2) into an R2A liquid culture medium for co-culture, and fermenting and culturing to a stable phase to obtain a complex microbial inoculant; the R2A liquid culture medium is prepared from 0.5 g/L of glucose, 0.5 g/L of peptone, 0.3 g/L of KH2PO4, 0.01 g/L of MgSO4, 0.3 g/L of sodium pyruvate, 0.5 g/L of acid hydrolyzed casein, 0.5 g/L of yeast extract and 0.5