Anti-S100A monoclonal antibody as well as preparation method and application thereof

The invention discloses an anti-S100A monoclonal antibody as well as a preparation method and application thereof. The anti-S100A monoclonal antibody provided by the invention comprises VHCDR1, VHCDR2 and VHCDR3 of which the amino acid sequences are as shown in SEQ ID NO: 1 to SEQ ID NO: 3, and VLCDR1, VLCDR2 and VLCDR3 of which the amino acid sequences are as shown in SEQ ID NO: 4 to SEQ ID NO: 6. Meanwhile, the invention also provides nucleotide sequences of the heavy chain variable region and the light chain variable region of the monoclonal antibody, so that a recombinant antibody can be prepared by utilizing a genetic engineering technology, the method is a standardized antibody production process, risk factors occurring in the traditional monoclonal antibody production and storage process are avoided, and the production efficiency is improved. The prepared recombinant antibody has the advantages of stable property, high experimental repeatability and the like, and the recombinant antibody can be subject