Rapid detection device for pymetrozine in agricultural products and detection method thereof

The invention discloses a rapid detection device for pymetrozine in agricultural products and a detection method of the rapid detection device, and relates to a hapten for immunization of pymetrozine, a hapten for coating, an antigen, an antibody, a colloidal gold chromatography detection device, pr...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Hauptverfasser: WU MINFU, WU MINHUA, ZHANG SHAOMIN, LU YUPING, ZHAN QINGMIN, WU HUILIN, GAO ROUMIN, LIN MUJIAN, LIN LIDONG, LIU KAOYU, LI SHA, FANG YUFANG, HE JINFENG, ZHOU HUANXIN
Format: Patent
Sprache:chi ; eng
Schlagworte:
Online-Zugang:Volltext bestellen
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:The invention discloses a rapid detection device for pymetrozine in agricultural products and a detection method of the rapid detection device, and relates to a hapten for immunization of pymetrozine, a hapten for coating, an antigen, an antibody, a colloidal gold chromatography detection device, preparation of the colloidal gold chromatography detection device and application of the colloidal gold chromatography detection device in pymetrozine detection. The detection method provided by the invention has the advantages of high sensitivity, strong specificity, low cost, simple operation, short detection time, long shelf life and the like. The invention provides the artificial antigen prepared by coupling the hapten for immunizing the pymetrozine with the carrier protein, and the specific antibody aiming at the pymetrozine generated by the body of an immunized experimental animal, the titer of the artificial antigen is high, the lowest detection limit of the pymetrozine is 0.1 mu g/mL, and the IC50 value is 10