Separation and purification method of coenzyme Q10

The invention discloses a separation and purification method of coenzyme Q10, which comprises the following steps: adding a pH regulator a into coenzyme Q10 fermentation liquor to regulate pH, and carrying out plate and frame filtration; hyphae are collected and squeezed, most of water is removed, and wet hyphae are obtained; the wet hyphae are taken, an extracting agent is added for pulping extraction, the extracting agent is selected from a combination of a solution a and a solution b, and the extracting agent a is selected from an aqueous solution which contains an antioxidant and has the pH of 8-12; the solvent b is selected from one or two of normal hexane and petroleum ether and contains a phase transfer extraction additive; by adding a certain proportion of the phase-transfer solid-phase extraction agent into the pulping extraction agent, high-efficiency extraction of wet hyphae can be realized, the extraction efficiency is improved, the extraction time is shortened, and the technical problem of low ef