PCR (Polymerase Chain Reaction) technology-based scallop variety identification method and specific primer thereof

The invention provides a scallop variety identification method based on a PCR (Polymerase Chain Reaction) technology and a specific primer thereof. The specific amplification primer is HZ, and the specific amplification primer is respectively composed of a left end primer sequence HZ-F: 5 '-CATTAACATGTGGTCATTGGC-3' and a right end primer sequence HZ-R: 5 '-AGTTTGTCCAACACCCAGGC-3'. The specific primer disclosed by the invention can be used for quickly, simply and conveniently identifying a plurality of scallop varieties such as bay scallop, argopecten purpuratus, first-filial generation of bay scallop and argopecten purpuratus, patinopecten yessoensis and chlamys farreri. According to the identification method provided by the invention, scallop variety identification can be realized only through PCR amplification, and the method is simple, efficient and low in cost. 本发明提供一种基于PCR技术的扇贝品种鉴定方法及其特异性引物。所述特异性扩增引物为HZ，分别由左端引物序列HZ-F:5'-CATTAACATCGTGGTCATTGGC-3'和右端引物序列HZ-R：5'-AGTTTGTCCAACACCCAGGC-3'组成。本发明的特异性引物能够快速简便地鉴别出