Straw mushroom low-temperature-resistant gene as well as screening method and application thereof

The invention provides a volvariella volvacea low-temperature-resistant gene. The gene sequence of the volvariella volvacea low-temperature-resistant gene is shown as any one of SEQ ID NO.1-3. The invention also provides an application of the volvariella volvacea low-temperature-resistant gene as a...

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Hauptverfasser: WANG YING, ZOU GEN, WAN JIANING, GONG MING, BAO DAPENG, WANG RUIJUAN, LI ZHENGPENG, ZHA LEI, GUO QIAN
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creator WANG YING
ZOU GEN
WAN JIANING
GONG MING
BAO DAPENG
WANG RUIJUAN
LI ZHENGPENG
ZHA LEI
GUO QIAN
description The invention provides a volvariella volvacea low-temperature-resistant gene. The gene sequence of the volvariella volvacea low-temperature-resistant gene is shown as any one of SEQ ID NO.1-3. The invention also provides an application of the volvariella volvacea low-temperature-resistant gene as a biomarker in screening low-temperature-resistant volvariella volvacea. The invention provides a method for screening novel low-temperature-resistant genes by utilizing low-temperature-resistant phenotypes of volvariella volvacea. The method comprises the following steps: constructing a novel volvariella volvacea low-temperature-resistant phenotype, performing gene expression sequencing on the volvariella volvacea low-temperature-resistant phenotype by adopting an absolute quantitative transcriptome technology, performing omics big data mining to obtain a volvariella volvacea low-temperature-resistant gene, and performing a yeast overexpression anti-freezing experiment on a target gene to verify the anti-freezing ap
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subjects BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS OR TEST PAPERS THEREFOR
COMPOSITIONS THEREOF
CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES
CULTURE MEDIA
ENZYMOLOGY
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROCESSES OF PREPARING SUCH COMPOSITIONS
PROCESSES USING MICROORGANISMS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
title Straw mushroom low-temperature-resistant gene as well as screening method and application thereof
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