Straw mushroom low-temperature-resistant gene as well as screening method and application thereof

The invention provides a volvariella volvacea low-temperature-resistant gene. The gene sequence of the volvariella volvacea low-temperature-resistant gene is shown as any one of SEQ ID NO.1-3. The invention also provides an application of the volvariella volvacea low-temperature-resistant gene as a...

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Bibliographische Detailangaben
Hauptverfasser:	WANG YING, ZOU GEN, WAN JIANING, GONG MING, BAO DAPENG, WANG RUIJUAN, LI ZHENGPENG, ZHA LEI, GUO QIAN
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention provides a volvariella volvacea low-temperature-resistant gene. The gene sequence of the volvariella volvacea low-temperature-resistant gene is shown as any one of SEQ ID NO.1-3. The invention also provides an application of the volvariella volvacea low-temperature-resistant gene as a biomarker in screening low-temperature-resistant volvariella volvacea. The invention provides a method for screening novel low-temperature-resistant genes by utilizing low-temperature-resistant phenotypes of volvariella volvacea. The method comprises the following steps: constructing a novel volvariella volvacea low-temperature-resistant phenotype, performing gene expression sequencing on the volvariella volvacea low-temperature-resistant phenotype by adopting an absolute quantitative transcriptome technology, performing omics big data mining to obtain a volvariella volvacea low-temperature-resistant gene, and performing a yeast overexpression anti-freezing experiment on a target gene to verify the anti-freezing ap