Promoter suitable for corynebacterium stagnation and application

The invention provides a promoter suitable for corynebacterium stagnation and application of the promoter. The nucleotide sequence of the promoter is shown as SEQ ID NO: 7, SEQ ID NO: 5 and/or SEQ ID NO: 6. The promoter is introduced into corynebacterium, so that high-level expression of corynebacterium arrest target protein is realized, and the problems of low strength, difficulty in expression of metabolites and proteins in coryneform bacteria and low expression efficiency of an existing promoter are solved, so that the purpose of further improving synthesis of the target protein is achieved; the expression level of GFP protein and alpha-amylase, which is 2.63 times and 2.36 times higher than that of the existing corynebacterium Psod promoter, is realized. 本发明提供了适用于停滞棒杆菌的启动子及应用。适用于停滞棒杆菌的启动子，其核苷酸序列如SEQ ID NO：7、SEQ ID NO：5和/或SEQ ID NO：6所示。本发明通过将启动子导入至棒杆菌内，实现停滞棒杆菌目的蛋白的高水平表达，克服了现有启动子强度低、棒状细菌中代谢物、蛋白表达难、表达效率低的问题，从而达到进一步提高目的蛋白合成的目的，实现了比现有棒杆菌Psod启动子高2.63倍与2.36倍的GFP蛋白及α-淀粉酶表达水平。