Method for simultaneously detecting amylose content and amylopectin content of pumpkin and application of method

The invention discloses a method for simultaneously detecting amylose and amylopectin contents of crops, which comprises the following steps: (1) detecting a to-be-detected solution by using a multifunctional microwell plate detector, with the amylose determination wavelength lambda1 being equal to 616nm, the amylose reference wavelength lambda2 being equal to 472nm, the amylopectin determination wavelength lambda3 being equal to 545nm and the amylopectin reference wavelength lambda4 being equal to 725nm; then respectively substituting detection results into a regression equation y1 =-0.8163 * 1 + 0.0747 of amylose and a regression equation y2 = 0.35 * 2 + 0.0123 of amylopectin; wherein y1 represents the light absorption value lambda2-lambda1 of the to-be-detected liquid, and x1 represents the amylose concentration of the to-be-detected liquid; y2 represents the light absorption value lambda3-lambda4 of the to-be-detected liquid, and x2 represents the amylopectin concentration of the to-be-detected liquid; an