Primer group and kit for pathogenic microorganism detection and application method thereof
The invention discloses a primer group and a kit for pathogenic microorganism detection and an application method of the primer group. The primer group comprises a universal bacterium detection primer group, a mycobacterium primer group, a mycoplasma spirochete detection primer group, a rickettsia d...
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| creator | WANG YING XI ZAIJUN FANG TAO LONG ZHICHENG ZHAO SHUYUN ZHANG JICHENG |
| description | The invention discloses a primer group and a kit for pathogenic microorganism detection and an application method of the primer group. The primer group comprises a universal bacterium detection primer group, a mycobacterium primer group, a mycoplasma spirochete detection primer group, a rickettsia detection primer group, a universal fungus detection primer group and a universal virus detection primer group, the specific primer sequences are as shown in SEQ ID NO. 1 to 98. The primer group provided by the invention can be used for simultaneously detecting bacteria, fungi and viruses, and is high in detection sensitivity and high in accuracy; besides, by matching with a primer group application method, the whole detection process can be completed within 4-6 hours, and rapid and instant detection of pathogenic microorganisms is realized.
本发明公开了用于病原微生物检测的引物组、试剂盒及其应用方法，所述引物组包括细菌通用检测引物组、分枝杆菌引物组、支原体螺旋体检测引物组、立克次体检测引物组、真菌通用检测引物组和病毒通用检测引物组，具体的引物序列如SEQ ID NO.1～98所示。本发明提供的引物组可以实现同时对细菌、真菌和病毒的检测，且检测灵敏度高，准确度高；另外，配合引物组应用方法，在 |
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本发明公开了用于病原微生物检测的引物组、试剂盒及其应用方法，所述引物组包括细菌通用检测引物组、分枝杆菌引物组、支原体螺旋体检测引物组、立克次体检测引物组、真菌通用检测引物组和病毒通用检测引物组，具体的引物序列如SEQ ID NO.1～98所示。本发明提供的引物组可以实现同时对细菌、真菌和病毒的检测，且检测灵敏度高，准确度高；另外，配合引物组应用方法，在</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS OR TEST PAPERS THEREFOR ; COMPOSITIONS THEREOF ; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ; CULTURE MEDIA ; ENZYMOLOGY ; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PROCESSES OF PREPARING SUCH COMPOSITIONS ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2023</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20230314&DB=EPODOC&CC=CN&NR=115786547A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25543,76293</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20230314&DB=EPODOC&CC=CN&NR=115786547A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>WANG YING</creatorcontrib><creatorcontrib>XI ZAIJUN</creatorcontrib><creatorcontrib>FANG TAO</creatorcontrib><creatorcontrib>LONG ZHICHENG</creatorcontrib><creatorcontrib>ZHAO SHUYUN</creatorcontrib><creatorcontrib>ZHANG JICHENG</creatorcontrib><title>Primer group and kit for pathogenic microorganism detection and application method thereof</title><description>The invention discloses a primer group and a kit for pathogenic microorganism detection and an application method of the primer group. The primer group comprises a universal bacterium detection primer group, a mycobacterium primer group, a mycoplasma spirochete detection primer group, a rickettsia detection primer group, a universal fungus detection primer group and a universal virus detection primer group, the specific primer sequences are as shown in SEQ ID NO. 1 to 98. The primer group provided by the invention can be used for simultaneously detecting bacteria, fungi and viruses, and is high in detection sensitivity and high in accuracy; besides, by matching with a primer group application method, the whole detection process can be completed within 4-6 hours, and rapid and instant detection of pathogenic microorganisms is realized.
本发明公开了用于病原微生物检测的引物组、试剂盒及其应用方法，所述引物组包括细菌通用检测引物组、分枝杆菌引物组、支原体螺旋体检测引物组、立克次体检测引物组、真菌通用检测引物组和病毒通用检测引物组，具体的引物序列如SEQ ID NO.1～98所示。本发明提供的引物组可以实现同时对细菌、真菌和病毒的检测，且检测灵敏度高，准确度高；另外，配合引物组应用方法，在</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS OR TEST PAPERS THEREFOR</subject><subject>COMPOSITIONS THEREOF</subject><subject>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PROCESSES OF PREPARING SUCH COMPOSITIONS</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2023</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNi7EKwjAQQLs4iPoP5wc4FK3tKkVxEgcnl3Ikl_SwyYVL_H-l-AFOjwfvLavnXTmQgld5J8Bo4cUFnCgkLKN4imwgsFER9Rg5B7BUyBSWOOeY0sQGZw_0XSyUkZTErauFwynT5sdVtb2cH_11R0kGygkNRSpDf6vrpu2OzaE97f9pPmHIO6c</recordid><startdate>20230314</startdate><enddate>20230314</enddate><creator>WANG YING</creator><creator>XI ZAIJUN</creator><creator>FANG TAO</creator><creator>LONG ZHICHENG</creator><creator>ZHAO SHUYUN</creator><creator>ZHANG JICHENG</creator><scope>EVB</scope></search><sort><creationdate>20230314</creationdate><title>Primer group and kit for pathogenic microorganism detection and application method thereof</title><author>WANG YING ; XI ZAIJUN ; FANG TAO ; LONG ZHICHENG ; ZHAO SHUYUN ; ZHANG JICHENG</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN115786547A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2023</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS OR TEST PAPERS THEREFOR</topic><topic>COMPOSITIONS THEREOF</topic><topic>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PROCESSES OF PREPARING SUCH COMPOSITIONS</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>WANG YING</creatorcontrib><creatorcontrib>XI ZAIJUN</creatorcontrib><creatorcontrib>FANG TAO</creatorcontrib><creatorcontrib>LONG ZHICHENG</creatorcontrib><creatorcontrib>ZHAO SHUYUN</creatorcontrib><creatorcontrib>ZHANG JICHENG</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>WANG YING</au><au>XI ZAIJUN</au><au>FANG TAO</au><au>LONG ZHICHENG</au><au>ZHAO SHUYUN</au><au>ZHANG JICHENG</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Primer group and kit for pathogenic microorganism detection and application method thereof</title><date>2023-03-14</date><risdate>2023</risdate><abstract>The invention discloses a primer group and a kit for pathogenic microorganism detection and an application method of the primer group. The primer group comprises a universal bacterium detection primer group, a mycobacterium primer group, a mycoplasma spirochete detection primer group, a rickettsia detection primer group, a universal fungus detection primer group and a universal virus detection primer group, the specific primer sequences are as shown in SEQ ID NO. 1 to 98. The primer group provided by the invention can be used for simultaneously detecting bacteria, fungi and viruses, and is high in detection sensitivity and high in accuracy; besides, by matching with a primer group application method, the whole detection process can be completed within 4-6 hours, and rapid and instant detection of pathogenic microorganisms is realized.
本发明公开了用于病原微生物检测的引物组、试剂盒及其应用方法，所述引物组包括细菌通用检测引物组、分枝杆菌引物组、支原体螺旋体检测引物组、立克次体检测引物组、真菌通用检测引物组和病毒通用检测引物组，具体的引物序列如SEQ ID NO.1～98所示。本发明提供的引物组可以实现同时对细菌、真菌和病毒的检测，且检测灵敏度高，准确度高；另外，配合引物组应用方法，在</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Primer group and kit for pathogenic microorganism detection and application method thereof |
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