Primer group and kit for pathogenic microorganism detection and application method thereof

The invention discloses a primer group and a kit for pathogenic microorganism detection and an application method of the primer group. The primer group comprises a universal bacterium detection primer group, a mycobacterium primer group, a mycoplasma spirochete detection primer group, a rickettsia detection primer group, a universal fungus detection primer group and a universal virus detection primer group, the specific primer sequences are as shown in SEQ ID NO. 1 to 98. The primer group provided by the invention can be used for simultaneously detecting bacteria, fungi and viruses, and is high in detection sensitivity and high in accuracy; besides, by matching with a primer group application method, the whole detection process can be completed within 4-6 hours, and rapid and instant detection of pathogenic microorganisms is realized. 本发明公开了用于病原微生物检测的引物组、试剂盒及其应用方法，所述引物组包括细菌通用检测引物组、分枝杆菌引物组、支原体螺旋体检测引物组、立克次体检测引物组、真菌通用检测引物组和病毒通用检测引物组，具体的引物序列如SEQ ID NO.1～98所示。本发明提供的引物组可以实现同时对细菌、真菌和病毒的检测，且检测灵敏度高，准确度高；另外，配合引物组应用方法，在