Saccharomyces cerevisiae engineering bacterium with high yield of santalol as well as construction method and application thereof

Saccharomyces cerevisiae engineering bacterium with high yield of santalol as well as construction method and application thereof

The invention is applicable to the field of microbial metabolism and synthetic biology, and provides a saccharomyces cerevisiae engineering strain with high yield of santalol as well as a construction method and application thereof, the saccharomyces cerevisiae engineering strain with high yield of...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: FU RONG, AN TIANYUE, LI DEFANG, WANG GUOLI
Format: Patent
Sprache:chi ; eng
Schlagworte:
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROCESSES USING MICROORGANISMS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Beschreibung
Zusammenfassung:The invention is applicable to the field of microbial metabolism and synthetic biology, and provides a saccharomyces cerevisiae engineering strain with high yield of santalol as well as a construction method and application thereof, the saccharomyces cerevisiae engineering strain with high yield of santalol takes saccharomyces cerevisiae as an original strain, overexpresses IME4, tHMGR, UPC2-1, SaSS, CYP736A167 and AtCPR1 genes, and knocks down ERG9 gene; wherein a promoter of the IME4 gene is replaced by a constitutive strong promoter PGK1p; the method comprises the following steps: integrating tHMGR and UPC2-1 genes into a TY3 site of a saccharomyces cerevisiae chromosome; a promoter of the ERG9 gene is replaced by a galactose inhibition type promoter HXT1p; the SaSS gene, the CYP736A167 gene and the At CPR1 gene are integrated to a TY2 site of a saccharomyces cerevisiae chromosome. The saccharomyces cerevisiae engineering bacteria constructed by the embodiment of the invention can be used for producing san