Construction and application of bacteriophage recombinant protein RecT-mediated bacillus licheniformis recombination system

The invention discloses construction and application of a bacteriophage recombinant protein RecT-mediated bacillus licheniformis recombination system, and belongs to the technical field of genetic engineering. According to the invention, a recombinant protein RecT from bacillus phage 049ML001 is excavated and identified, and a rhamnose induced promoter Prha is utilized to construct a homologous recombination system expressed under recombinase conditions. And the recombination efficiency of the system is further improved by optimizing the action condition of the recombinase. According to the invention, a bacteriophage recombinant protein mediated recombination system is constructed in the bacillus licheniformis for the first time, gene knockout is realized, and an effective technical means is provided for metabolic engineering modification of the bacillus licheniformis. 本发明公开了一种噬菌体重组蛋白RecT介导的地衣芽孢杆菌重组系统的构建及应用，属于基因工程技术领域。本发明挖掘并鉴定了来源于芽孢杆菌噬菌体(Bacillus phage)049ML001的重组蛋白RecT，利用鼠李糖诱导启动子Prha构建了重组酶条件表达的同源重组系统。进一步通过优化