Human umbilical cord mesenchymal stem cell osteogenic induced differentiation culture medium and application thereof

The invention provides a human umbilical cord mesenchymal stem cell osteogenesis induced differentiation culture medium and application thereof, and belongs to the technical field of stem cells. The invention provides a culture medium composition for osteogenic induced differentiation culture of hum...

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Hauptverfasser: XU DAOJUN, ZHAO QIONGYING, WU MAOYOU, ZHOU QIAO, LI MEILING, GU SONGHAI
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention provides a human umbilical cord mesenchymal stem cell osteogenesis induced differentiation culture medium and application thereof, and belongs to the technical field of stem cells. The invention provides a culture medium composition for osteogenic induced differentiation culture of human umbilical cord mesenchymal stem cells. The culture medium composition comprises a basic culture medium, an inducing factor and an additive factor, wherein the basic culture medium comprises fetal calf serum FBS, penicillin, streptomycin and a serum-free phenol red-free mesenchymal stem cell culture medium; the induction factors comprise dexamethasone, beta-sodium glycerophosphate and vitamin C; the addition factors comprise 17 beta-estradiol and psoralen. The culture medium disclosed by the invention can be used for culturing the human umbilical cord mesenchymal stem cells to be differentiated into the osteoblasts, the culture time of the human umbilical cord mesenchymal stem cells to be differentiated into the