Culture medium and culture method suitable for Vero cell full-suspension large-scale culture
The invention discloses a culture medium and a culture method suitable for Vero cell full-suspension large-scale culture. The culture medium is a serum-free culture medium specially designed, researched and developed according to the growth and metabolism characteristics of Vero suspension cells and...
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Format: | Patent |
Sprache: | chi ; eng |
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Zusammenfassung: | The invention discloses a culture medium and a culture method suitable for Vero cell full-suspension large-scale culture. The culture medium is a serum-free culture medium specially designed, researched and developed according to the growth and metabolism characteristics of Vero suspension cells and different multiplication capacities of the Vero suspension cells when the Vero suspension cells are horizontally cultured on a reactor and on a shaking table in combination with different-scale culture differences, and the serum-free culture medium comprises the following components: amino acid, protein, vitamins, inorganic salts, serum substitution factors, auxiliary components and the like. Besides, according to cell growth characteristics, control parameters of a full-suspension culture reactor are determined, so that the VERO cell culture density is greatly improved, the cell multiplication rate reaches 10 times after 48 hours of culture, the cell production cycle is shortened by half, meanwhile, the productio |
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