Bacillus tropicalis CSN-15 strain and application thereof

The invention belongs to the technical field of bioengineering, provides a microbial strain for producing chitosanase, and particularly relates to bacillus tropicalis, and a method for producing chitosanase by using the bacillus tropicalis and producing chitosan oligosaccharide by using the chitosanase. According to the invention, a method for producing chitosanase and preparing chitosan oligosaccharide by fermentation is further researched by researching a chitosanase-producing strain CSN-15 obtained by screening a sea mud sample. Compared with the chitosanase which is commonly used at present, the chitosanase produced by the CSN-15 strain is higher in activity, the enzyme activity is 4.28 U/ml, and the CSN-15 strain can be applied to degradation of the chitosanase, and is shorter in catalysis time, wider in application range and suitable for industrial production of chitosan oligosaccharide. 本发明属于生物工程技术领域，提供了一株产壳聚糖酶的微生物菌株，特别是涉及一株热带芽孢杆菌，及利用该菌生产壳聚糖酶和利用该酶生产壳寡糖的方法。本发明通过对从海泥样品筛选，对其中得到的产壳聚糖酶菌株CSN-15进行研究，进一步研究了发酵生