Fusion protein of porcine reproductive and respiratory syndrome virus and application thereof

The invention discloses a porcine reproductive and respiratory syndrome virus fusion protein and application thereof, and belongs to the technical field of biology. The fusion protein provided by the invention is obtained by connecting a plurality of antigen epitopes of GP5 proteins of different strains of porcine reproductive and respiratory syndrome virus in series, and comprises or consists of an amino acid sequence as shown in SEQ ID NO: 1. The expression quantity of the fusion protein provided by the invention in a prokaryotic expression system is obviously improved, and an antibody detection kit prepared from the fusion protein can be used for detecting the porcine reproductive and respiratory syndrome virus antibody in a broad-spectrum manner, so that missing detection caused by difference of antigenicity is avoided. 本发明公开一种猪繁殖与呼吸综合征病毒的融合蛋白及其应用，属于生物技术领域。本发明提供的融合蛋白为猪繁殖与呼吸综合征病毒不同毒株的GP5蛋白的多个抗原表位经串联得到，其包含SEQ ID NO:1所示的氨基酸序列或由其组成。本发明提供的融合蛋白在原核表达系统中的表达量明显提高，且由其制备的抗体检测试剂盒可以广谱性检测猪繁殖与呼吸综合征病毒抗体，避免因抗原性的差异而导致的漏检。