Method for analyzing component proportion of ultra-low molecular weight hyaluronic acid

The invention relates to a method for analyzing the proportion of components in ultra-low molecular weight hyaluronic acid through anion exchange chromatography. According to the anion exchange chromatography, a ZORBAX SAX chromatographic column or a Spherisorb SAX chromatographic column is adopted; gradient elution is carried out by adopting a mobile phase consisting of water and a sulfate aqueous solution. The analysis method disclosed by the invention can avoid interference of a matrix on detection, and meanwhile, the stationary phase is lower in price, so that the cost can be greatly saved; the used mobile phase is common in composition, simple to prepare, trouble-saving and labor-saving; the mobile phase operation time is short, and the operation is time-saving and convenient, so that the analysis method is suitable for popularization and application. 本发明涉及通过阴离子交换色谱法分析超低分子量透明质酸中组分比例，所述阴离子交换色谱法采用ZORBAX SAX色谱柱或Spherisorb SAX色谱柱；采用水和硫酸盐水溶液组成的流动相进行梯度洗脱。本发明的分析方法能避免基质对检测的干扰，同时固定相价格更低，可大大节省成本；所使用的流动相组成常见、配制简单，省