Enzyme mutant and application thereof

The invention relates to the technical field of biological catalysis, in particular to an enzyme mutant and application thereof. The invention provides a mutant of D-amino acid oxidase derived from Sporidiobolus salmoninus, wherein the mutation sites comprise N54V, C56N, F58H, M216S, R116V and/or K3...

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Bibliographische Detailangaben
Hauptverfasser:	LOU YIYUAN, LIU MINGHUA, HE JUNGUANG, REN BUFAN, QIN LONG, WU YANQING, JIANG SHENGBAO, CHEN XIANHUI, ZHOU SHUGUANG, XU YAQING, LIU ZHIWEI, HU BEIBEI
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention relates to the technical field of biological catalysis, in particular to an enzyme mutant and application thereof. The invention provides a mutant of D-amino acid oxidase derived from Sporidiobolus salmoninus, wherein the mutation sites comprise N54V, C56N, F58H, M216S, R116V and/or K319T. The mutant has the characteristics of high enzyme activity and high conversion rate, can be used for efficiently preparing 2-carbonyl-4-[hydroxyl (methyl) phosphonyl] butyric acid, and is high in product yield. 本发明涉及生物催化技术领域，特别涉及酶突变体及其应用。本发明提供了来源于鲑色锁掷酵母的D-氨基酸氧化酶的突变体，突变位点包括N54V、C56N、F58H、M216S、R116V和/或K319T。该突变体具有高酶活、高转化率的特点，可高效制备2-羰基-4-[羟基(甲基)膦酰基]丁酸，产物收率高。