Fusion protein capable of simultaneously expressing peste des petits ruminants H and F proteins, double-gene subunit vaccine and preparation method
The invention provides a fusion protein capable of simultaneously expressing peste des petits ruminants H and F proteins, a double-gene subunit vaccine and a preparation method, and relates to the technical field of veterinary biological products. The fusion protein is subjected to enzyme digestion...
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| creator | ZHANG JINLONG WANG JIAFU ZHANG MANXIN DONG PENG LI XUEFENG KANG BIN WU YUMEI ZHAO BINGWU |
| description | The invention provides a fusion protein capable of simultaneously expressing peste des petits ruminants H and F proteins, a double-gene subunit vaccine and a preparation method, and relates to the technical field of veterinary biological products. The fusion protein is subjected to enzyme digestion and in-vitro refolding to obtain H protein in a dimer form and F protein in a trimer form, and the H protein and the F protein are mixed and emulsified with an adjuvant to form the double-gene subunit vaccine. According to the present invention, the two protective antigens of peste des petits ruminants can be simultaneously produced through the one-time expression process, the purification filler and the enzyme digestion enzyme can be repeatedly used, the purification process is simple, the production cost is low, the antigen purity is high, the immune side reaction is effectively reduced, and the immune protection of the double-gene subunit vaccine is comprehensive.
本发明提供了一种同时表达小反刍兽疫H和F蛋白的融合蛋白、双基因亚单位疫苗及制备方法，涉及兽用生物 |
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本发明提供了一种同时表达小反刍兽疫H和F蛋白的融合蛋白、双基因亚单位疫苗及制备方法，涉及兽用生物</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; HUMAN NECESSITIES ; HYGIENE ; MEDICAL OR VETERINARY SCIENCE ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; ORGANIC CHEMISTRY ; PEPTIDES ; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2023</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20230310&DB=EPODOC&CC=CN&NR=115772231A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25543,76294</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20230310&DB=EPODOC&CC=CN&NR=115772231A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>ZHANG JINLONG</creatorcontrib><creatorcontrib>WANG JIAFU</creatorcontrib><creatorcontrib>ZHANG MANXIN</creatorcontrib><creatorcontrib>DONG PENG</creatorcontrib><creatorcontrib>LI XUEFENG</creatorcontrib><creatorcontrib>KANG BIN</creatorcontrib><creatorcontrib>WU YUMEI</creatorcontrib><creatorcontrib>ZHAO BINGWU</creatorcontrib><title>Fusion protein capable of simultaneously expressing peste des petits ruminants H and F proteins, double-gene subunit vaccine and preparation method</title><description>The invention provides a fusion protein capable of simultaneously expressing peste des petits ruminants H and F proteins, a double-gene subunit vaccine and a preparation method, and relates to the technical field of veterinary biological products. The fusion protein is subjected to enzyme digestion and in-vitro refolding to obtain H protein in a dimer form and F protein in a trimer form, and the H protein and the F protein are mixed and emulsified with an adjuvant to form the double-gene subunit vaccine. According to the present invention, the two protective antigens of peste des petits ruminants can be simultaneously produced through the one-time expression process, the purification filler and the enzyme digestion enzyme can be repeatedly used, the purification process is simple, the production cost is low, the antigen purity is high, the immune side reaction is effectively reduced, and the immune protection of the double-gene subunit vaccine is comprehensive.
本发明提供了一种同时表达小反刍兽疫H和F蛋白的融合蛋白、双基因亚单位疫苗及制备方法，涉及兽用生物</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>HUMAN NECESSITIES</subject><subject>HYGIENE</subject><subject>MEDICAL OR VETERINARY SCIENCE</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>ORGANIC CHEMISTRY</subject><subject>PEPTIDES</subject><subject>PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2023</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNjrEKwkAQRNNYiPoPa2-KJEhqCYZUVvZhTTbx4LJ3ZPdEv8Mf9gLaW80MPB6zTt51EOMY_OyUDEOHHm-WwA0gZgpWkckFsS-gp59JxPAInkQJepLY1KjAHCbDyLE1gNxD_fPJAXoXojAdiQkk3AIbhQd2nYl7YaPV44y6vJhI767fJqsBrdDum5tkX5-vVZOSdy2Jxy66tK0uWXYsyzwvslPxD_MBCkVQ9w</recordid><startdate>20230310</startdate><enddate>20230310</enddate><creator>ZHANG JINLONG</creator><creator>WANG JIAFU</creator><creator>ZHANG MANXIN</creator><creator>DONG PENG</creator><creator>LI XUEFENG</creator><creator>KANG BIN</creator><creator>WU YUMEI</creator><creator>ZHAO BINGWU</creator><scope>EVB</scope></search><sort><creationdate>20230310</creationdate><title>Fusion protein capable of simultaneously expressing peste des petits ruminants H and F proteins, double-gene subunit vaccine and preparation method</title><author>ZHANG JINLONG ; WANG JIAFU ; ZHANG MANXIN ; DONG PENG ; LI XUEFENG ; KANG BIN ; WU YUMEI ; ZHAO BINGWU</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN115772231A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2023</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>HUMAN NECESSITIES</topic><topic>HYGIENE</topic><topic>MEDICAL OR VETERINARY SCIENCE</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>ORGANIC CHEMISTRY</topic><topic>PEPTIDES</topic><topic>PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>ZHANG JINLONG</creatorcontrib><creatorcontrib>WANG JIAFU</creatorcontrib><creatorcontrib>ZHANG MANXIN</creatorcontrib><creatorcontrib>DONG PENG</creatorcontrib><creatorcontrib>LI XUEFENG</creatorcontrib><creatorcontrib>KANG BIN</creatorcontrib><creatorcontrib>WU YUMEI</creatorcontrib><creatorcontrib>ZHAO BINGWU</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>ZHANG JINLONG</au><au>WANG JIAFU</au><au>ZHANG MANXIN</au><au>DONG PENG</au><au>LI XUEFENG</au><au>KANG BIN</au><au>WU YUMEI</au><au>ZHAO BINGWU</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Fusion protein capable of simultaneously expressing peste des petits ruminants H and F proteins, double-gene subunit vaccine and preparation method</title><date>2023-03-10</date><risdate>2023</risdate><abstract>The invention provides a fusion protein capable of simultaneously expressing peste des petits ruminants H and F proteins, a double-gene subunit vaccine and a preparation method, and relates to the technical field of veterinary biological products. The fusion protein is subjected to enzyme digestion and in-vitro refolding to obtain H protein in a dimer form and F protein in a trimer form, and the H protein and the F protein are mixed and emulsified with an adjuvant to form the double-gene subunit vaccine. According to the present invention, the two protective antigens of peste des petits ruminants can be simultaneously produced through the one-time expression process, the purification filler and the enzyme digestion enzyme can be repeatedly used, the purification process is simple, the production cost is low, the antigen purity is high, the immune side reaction is effectively reduced, and the immune protection of the double-gene subunit vaccine is comprehensive.
本发明提供了一种同时表达小反刍兽疫H和F蛋白的融合蛋白、双基因亚单位疫苗及制备方法，涉及兽用生物</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS SPIRITS VINEGAR WINE |
| title | Fusion protein capable of simultaneously expressing peste des petits ruminants H and F proteins, double-gene subunit vaccine and preparation method |
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