Fusion protein capable of simultaneously expressing peste des petits ruminants H and F proteins, double-gene subunit vaccine and preparation method

The invention provides a fusion protein capable of simultaneously expressing peste des petits ruminants H and F proteins, a double-gene subunit vaccine and a preparation method, and relates to the technical field of veterinary biological products. The fusion protein is subjected to enzyme digestion and in-vitro refolding to obtain H protein in a dimer form and F protein in a trimer form, and the H protein and the F protein are mixed and emulsified with an adjuvant to form the double-gene subunit vaccine. According to the present invention, the two protective antigens of peste des petits ruminants can be simultaneously produced through the one-time expression process, the purification filler and the enzyme digestion enzyme can be repeatedly used, the purification process is simple, the production cost is low, the antigen purity is high, the immune side reaction is effectively reduced, and the immune protection of the double-gene subunit vaccine is comprehensive. 本发明提供了一种同时表达小反刍兽疫H和F蛋白的融合蛋白、双基因亚单位疫苗及制备方法，涉及兽用生物