Method for identifying cyprinid fish gelatin

The invention relates to the technical field of gelatin identification methods, in particular to a cyprinid fish gelatin source identification method. The invention aims to provide a method for identifying cyprinid fish gelatin based on consensus characteristic and unique characteristic polypeptide...

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Hauptverfasser: TANG XUEQI, TU ZONGCAI, HU ZIZI, JIANG WENLI, SHA XIAOMEI
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention relates to the technical field of gelatin identification methods, in particular to a cyprinid fish gelatin source identification method. The invention aims to provide a method for identifying cyprinid fish gelatin based on consensus characteristic and unique characteristic polypeptide database analysis. An identification method of cyprinid fish gelatin comprises the following steps: extracting an amino acid sequence of cyprinid fish type I collagen from an NCBI (National Center of Biotechnology Information) database, and establishing a common and unique theoretical characteristic polypeptide database of the cyprinid fish gelatin by utilizing protein sequence comparison software and collagenase-trypsin combination to simulate digestion. And common and unique characteristic polypeptides different from cyprinid fish gelatin are identified by adopting an HPLC-MS/MS mass spectrometry technology, so that the accuracy and authenticity of traceability identification of cyprinid fish gelatin are improved