Kit for detecting residual DNA of HEK293 cells by qPCR method
The invention belongs to the technical field of gene detection, and particularly relates to a kit for detecting residual DNA (deoxyribonucleic acid) of HEK293 cells by a qPCR (quantitative polymerase chain reaction) method. The invention discloses a qPCR (quantitative polymerase chain reaction) meth...
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| creator | NIU MAOMAO ZHAO GUIFEN CAO KEKE |
| description | The invention belongs to the technical field of gene detection, and particularly relates to a kit for detecting residual DNA (deoxyribonucleic acid) of HEK293 cells by a qPCR (quantitative polymerase chain reaction) method. The invention discloses a qPCR (quantitative polymerase chain reaction) method HEK293 cell residual DNA (deoxyribonucleic acid) detection kit, which comprises a plurality of reagent bottles respectively filled with PCR amplification reaction liquid, a negative quality control, a positive quality control and a quantitative reference and sealed with covers, and a packaging box for separating and intensively packaging the reagent bottles, and is characterized in that the PCR amplification reaction liquid is filled with the negative quality control, the positive quality control and the quantitative reference; the PCR amplification reaction liquid contains PremixEx Taq, a DNA (deoxyribonucleic acid) template, a Negitivetron, a specific primer and a probe, and the specific primer is used for det |
| format | Patent |
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The invention discloses a qPCR (quantitative polymerase chain reaction) method HEK293 cell residual DNA (deoxyribonucleic acid) detection kit, which comprises a plurality of reagent bottles respectively filled with PCR amplification reaction liquid, a negative quality control, a positive quality control and a quantitative reference and sealed with covers, and a packaging box for separating and intensively packaging the reagent bottles, and is characterized in that the PCR amplification reaction liquid is filled with the negative quality control, the positive quality control and the quantitative reference; the PCR amplification reaction liquid contains PremixEx Taq, a DNA (deoxyribonucleic acid) template, a Negitivetron, a specific primer and a probe, and the specific primer is used for det</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS OR TEST PAPERS THEREFOR ; COMPOSITIONS THEREOF ; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ; CULTURE MEDIA ; ENZYMOLOGY ; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PROCESSES OF PREPARING SUCH COMPOSITIONS ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2023</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20230307&DB=EPODOC&CC=CN&NR=115747344A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,309,781,886,25569,76552</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20230307&DB=EPODOC&CC=CN&NR=115747344A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>NIU MAOMAO</creatorcontrib><creatorcontrib>ZHAO GUIFEN</creatorcontrib><creatorcontrib>CAO KEKE</creatorcontrib><title>Kit for detecting residual DNA of HEK293 cells by qPCR method</title><description>The invention belongs to the technical field of gene detection, and particularly relates to a kit for detecting residual DNA (deoxyribonucleic acid) of HEK293 cells by a qPCR (quantitative polymerase chain reaction) method. The invention discloses a qPCR (quantitative polymerase chain reaction) method HEK293 cell residual DNA (deoxyribonucleic acid) detection kit, which comprises a plurality of reagent bottles respectively filled with PCR amplification reaction liquid, a negative quality control, a positive quality control and a quantitative reference and sealed with covers, and a packaging box for separating and intensively packaging the reagent bottles, and is characterized in that the PCR amplification reaction liquid is filled with the negative quality control, the positive quality control and the quantitative reference; the PCR amplification reaction liquid contains PremixEx Taq, a DNA (deoxyribonucleic acid) template, a Negitivetron, a specific primer and a probe, and the specific primer is used for det</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS OR TEST PAPERS THEREFOR</subject><subject>COMPOSITIONS THEREOF</subject><subject>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PROCESSES OF PREPARING SUCH COMPOSITIONS</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2023</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZLD1zixRSMsvUkhJLUlNLsnMS1coSi3OTClNzFFw8XNUyE9T8HD1NrI0VkhOzckpVkiqVCgMcA5SyE0tychP4WFgTUvMKU7lhdLcDIpuriHOHrqpBfnxqcUFicmpeakl8c5-hoam5ibmxiYmjsbEqAEAESstzg</recordid><startdate>20230307</startdate><enddate>20230307</enddate><creator>NIU MAOMAO</creator><creator>ZHAO GUIFEN</creator><creator>CAO KEKE</creator><scope>EVB</scope></search><sort><creationdate>20230307</creationdate><title>Kit for detecting residual DNA of HEK293 cells by qPCR method</title><author>NIU MAOMAO ; ZHAO GUIFEN ; CAO KEKE</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN115747344A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2023</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS OR TEST PAPERS THEREFOR</topic><topic>COMPOSITIONS THEREOF</topic><topic>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PROCESSES OF PREPARING SUCH COMPOSITIONS</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>NIU MAOMAO</creatorcontrib><creatorcontrib>ZHAO GUIFEN</creatorcontrib><creatorcontrib>CAO KEKE</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>NIU MAOMAO</au><au>ZHAO GUIFEN</au><au>CAO KEKE</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Kit for detecting residual DNA of HEK293 cells by qPCR method</title><date>2023-03-07</date><risdate>2023</risdate><abstract>The invention belongs to the technical field of gene detection, and particularly relates to a kit for detecting residual DNA (deoxyribonucleic acid) of HEK293 cells by a qPCR (quantitative polymerase chain reaction) method. The invention discloses a qPCR (quantitative polymerase chain reaction) method HEK293 cell residual DNA (deoxyribonucleic acid) detection kit, which comprises a plurality of reagent bottles respectively filled with PCR amplification reaction liquid, a negative quality control, a positive quality control and a quantitative reference and sealed with covers, and a packaging box for separating and intensively packaging the reagent bottles, and is characterized in that the PCR amplification reaction liquid is filled with the negative quality control, the positive quality control and the quantitative reference; the PCR amplification reaction liquid contains PremixEx Taq, a DNA (deoxyribonucleic acid) template, a Negitivetron, a specific primer and a probe, and the specific primer is used for det</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Kit for detecting residual DNA of HEK293 cells by qPCR method |
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