Kit for detecting residual DNA of HEK293 cells by qPCR method

The invention belongs to the technical field of gene detection, and particularly relates to a kit for detecting residual DNA (deoxyribonucleic acid) of HEK293 cells by a qPCR (quantitative polymerase chain reaction) method. The invention discloses a qPCR (quantitative polymerase chain reaction) meth...

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Bibliographische Detailangaben
Hauptverfasser: NIU MAOMAO, ZHAO GUIFEN, CAO KEKE
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention belongs to the technical field of gene detection, and particularly relates to a kit for detecting residual DNA (deoxyribonucleic acid) of HEK293 cells by a qPCR (quantitative polymerase chain reaction) method. The invention discloses a qPCR (quantitative polymerase chain reaction) method HEK293 cell residual DNA (deoxyribonucleic acid) detection kit, which comprises a plurality of reagent bottles respectively filled with PCR amplification reaction liquid, a negative quality control, a positive quality control and a quantitative reference and sealed with covers, and a packaging box for separating and intensively packaging the reagent bottles, and is characterized in that the PCR amplification reaction liquid is filled with the negative quality control, the positive quality control and the quantitative reference; the PCR amplification reaction liquid contains PremixEx Taq, a DNA (deoxyribonucleic acid) template, a Negitivetron, a specific primer and a probe, and the specific primer is used for det