Method for infecting rhododendron flower buds with agrobacterium tumefaciens

The invention belongs to the technical field of biology, and relates to a method for infecting rhododendron flower buds by agrobacterium. According to the method, reference is provided for other ornamental flowers by infecting the azalea flower buds with agrobacterium for the first time, and in the implementation process, compared with a leaf disc method and a gene gun method, the transformation method has the advantages of being easy to operate, low in cost, good in transformation effect and the like; the test time is greatly shortened through non-tissue culture transgenosis, and the stable inheritance effect can be achieved through the method that the azalea flower buds are infected with the agrobacterium tumefaciens. 本发明属于生物技术领域，涉及一种农杆菌侵染杜鹃花花蕾的方法。本发明首次利用农杆菌侵染杜鹃花花蕾为其他观赏性花卉提供参考，在实施过程中转化方法较叶盘法和基因枪法具有操作简单、成本低和转化效果好等优点；本发明非组织培养转基因大大缩短了试验时间，利用农杆菌侵染杜鹃花花蕾的方法可以达到稳定遗传的效果。