Large-system and high-efficiency nucleic acid extraction kit

The invention discloses a large-system and high-efficiency nucleic acid extraction kit which comprises a lysis solution, a washing solution 1, a washing solution 2, a washing solution 3, an eluent, a protease K solution and a magnetic bead mixed solution. By designing a kit system, the problems that...

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Hauptverfasser: WANG KANGYOU, GUO JUNHUI, LI HONGDONG, BU ZHENZHONG, LI MING
Format: Patent
Sprache:chi ; eng
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Beschreibung
Zusammenfassung:The invention discloses a large-system and high-efficiency nucleic acid extraction kit which comprises a lysis solution, a washing solution 1, a washing solution 2, a washing solution 3, an eluent, a protease K solution and a magnetic bead mixed solution. By designing a kit system, the problems that an existing kit is small in sample extraction system, low in sensitivity and complex in operation are solved. The kit system is simple in overall operation, nucleic acid extraction of a single-hole 1mL sample can be achieved in the nucleic acid extraction process, and large-system nucleic acid extraction is achieved. The kit is relatively high in nucleic acid extraction sensitivity and extraction efficiency. 本发明公开了一种大体系、高效率核酸提取试剂盒,包括裂解液、洗涤液1、洗涤液2、洗涤液3、洗脱液、蛋白酶K溶液和磁珠混合液。本发明通过对试剂盒体系进行设计,解决了现有试剂盒提取样本体系小,灵敏度低,操作复杂的问题。该试剂盒体系整体操作简单,在核酸提取过程中,可实现单孔1mL样本的核酸提取,实现了大体系核酸提取。该试剂盒核酸提取灵敏度及提取效率均较高。