Preparation and application of echinococcus canadensis EC95 protein

The invention discloses a preparation method and an application of an echinococcus canadensis EC95 protein. The recombinant protein comprises a dEC95 amino acid sequence; the dEC95 amino acid sequence is a modified amino acid sequence obtained by deleting an N-terminal signal peptide region and a C-terminal transmembrane region from EC95 of echinococcus canadensis; the sequence of the EC95 gene is as shown in SEQ ID NO. 1, and the amino acid sequence of the EC95 gene is as shown in SEQ ID NO. 2. The method comprises the following steps: modifying EC95 in the Canadian echinococcosis, and preparing recombinant expression plasmids and recombinant escherichia coli engineering bacteria; the fusion protein expressed by the recombinant escherichia coli engineering bacteria has the advantages of high purity, good solubility, convenience in purification, high biological activity and the like. 本发明公开了一种加拿大棘球蚴EC95蛋白的制备及应用；所述重组蛋白包括dEC95氨基酸序列；所述dEC95氨基酸序列为加拿大棘球绦虫的EC95删去N端的信号肽区域及C端的跨膜区，得到的修饰后的氨基酸序列；所述EC95基因序列如SEQ ID NO.1所示，