Subunit vaccine for Canadian echinococcosis as well as preparation method and application of subunit vaccine

The invention discloses a subunit vaccine for resisting Canadian echinococcosis infection and a preparation method of the subunit vaccine. The EC95 antigen protein comprises an immunogen and a pharmaceutically acceptable carrier; wherein the amino acid sequence of the dEC95 protein is as shown in SEQ ID No. 1 or a degenerate sequence of the SEQ ID No. 1. At present, no vaccine for resisting Canadian echinococcosis infection exists, 2dEC95 protein is adopted as an antigen, and the prepared subunit vaccine for resisting Canadian echinococcosis infection has the advantages of low production cost, simple production process, safety, high efficiency, low cost and the like; even if the content of dEC95 protein antigen in the anti-Canadian echinococcosis subunit vaccine is only 40 [mu] g/ml, the antibody titer of 1: 128 or above can be achieved in the fourth week after secondary immunization, and the long-term high-titer antibody titer can be maintained. 本发明公开了一种抗加拿大棘球蚴感染的亚单位疫苗及其制备方法；包含免疫原的EC95抗原蛋白和药学上可以接受的载体；其中，dEC9