Tissue culture method of Mongolian oak

The invention provides a Mongolian oak tissue culture method. The Mongolian oak tissue culture method comprises the following steps: selection of explants, sterilization treatment of the explants, induction culture of the explants, multiplication culture of the explants, rooting culture of the explants, and seedling hardening and domestication of aseptic seedlings. According to the Mongolian oak tissue culture method, early-stage disinfection means such as dishwashing liquid soaking, 75% alcohol wiping, 0.1% mercuric chloride disinfection and sterile water flushing are adopted in explant sterilization treatment, the explant sterilization effect is better, it is guaranteed that the problems of pollution, browning, yellowing and the like are avoided in the culture period, and the Mongolian oak tissue culture effect is improved. 本发明提供一种蒙古栎的组织培养方法。一种蒙古栎的组织培养方法包括以下步骤：外植体的选择、外植体的灭菌处理、外植体的诱导培养、外植体的增殖培养、外植体的生根培养、无菌苗的炼苗驯化。本发明通过在外植体的灭菌处理中采用洗洁精浸泡、75％酒精擦拭、0.1％氯化汞消毒、无菌水冲洗等前期消毒手段，对外植体的灭菌效果更好，保证培养期间避免出现受污染、褐化、黄化等问题，提高蒙古栎的组织