RT-LAMP detection primer, detection method and kit for tobacco susceptible South America red pepper vein mottle virus

The invention discloses an RT-LAMP (Reverse Transcription Loop-Mediated Isothermal Amplification) detection primer, a detection method and a kit for tobacco susceptible South America red pepper vein mottle virus. The RT-LAMP detection primer is CH-CP-3 or CH-CP-4. The invention provides an RT-LAMP (...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Hauptverfasser:	LIU YATING, ZHANG JUNLEI, MA JUNHONG, MA JUNMEI, LU CANHUA, GE XIAOTONG, XIA ZHENYUAN, ZHAO ZHENGTING, JIANG NING
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
Online-Zugang:	Volltext bestellen
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	The invention discloses an RT-LAMP (Reverse Transcription Loop-Mediated Isothermal Amplification) detection primer, a detection method and a kit for tobacco susceptible South America red pepper vein mottle virus. The RT-LAMP detection primer is CH-CP-3 or CH-CP-4. The invention provides an RT-LAMP (Reverse Transcription Loop-Mediated Isothermal Amplification) detection primer pair aiming at the South America red pepper vein mottle virus (ChiVMV), by adopting the primer pair, a false positive result can be effectively avoided, the detection accuracy is improved, and meanwhile, the detection time can be effectively shortened to 50 minutes. In addition, RT-LAMP detection can directly judge a result (visually interpret the result) through visual observation, the sensitivity is 100 times that of RT-PCR, the RT-LAMP detection method is simpler and easier than RT-PCR, and the requirements of scientific research, grassroots units, simple and crude sites and the like on virus detection can be met. 本申请公开了一种烟草易感南美红辣椒脉斑驳