Primer and probe for rapidly detecting tobacco target leaf spot pathogen, application, kit and tobacco target leaf spot pathogen rapid detection method

The invention provides a primer and a probe for rapidly detecting tobacco target leaf spot germs, application, a kit and a rapid detection method for the tobacco target leaf spot germs, and relates to the technical field of tobacco prevention and control. The primer and the probe for rapidly detecting the tobacco target leaf spot germs are optimized and screened out through tests and have high specificity on the tobacco target leaf spot germs, and amplification products of the primer and the probe are single strips and free of non-specific amplification and obvious primer dimers. The primer and the probe for rapidly detecting the tobacco target leaf spot pathogen are used for performing RPA amplification on the target gene of the tobacco target leaf spot pathogen, and a lateral flow chromatography test strip is used for performing visual detection on the target gene, so that the primer and the probe have the advantages of high sensitivity, good specificity and short operation time. 本申请提供用于快速检测烟草靶斑病菌的引物和探针及应用、