Construction method and application of streptomyces double-promoter inducible shuttle vector

The invention belongs to the technical field of gene engineering, relates to a construction method and application of a streptomyces double-promoter inducible shuttle vector, and aims to provide a theoretical research basis for protein production, and the construction method comprises the following steps: inserting Kpn I and Spe I restriction enzyme cutting sites in a plasmid pSET152, inserting a constitutive strong promoter ermE * and an inducible promoter tipA, and carrying out screening to obtain the streptomyces double-promoter inducible shuttle vector. A Bsa I restriction site is introduced, so that a double-promoter induced shuttle vector pSETTK is prepared; the related application method comprises the following steps: adding a fragment of a signal peptide fusion target gene to a shuttle vector pSETTK to form an expression plasmid; streptomyces is used as a host, and finally inducible expression of target protein is realized. The shuttle vector pSETTK disclosed by the invention has the advantages that t