Bacterial laccase LacP.E., coding gene, application of bacterial laccase LacP.E. And feed additive

The invention relates to bacterial laccase LacP.E., a coding gene and application thereof, and a feed additive, and belongs to the technical field of enzyme engineering. The bacterial laccase LacP.E. is obtained by constructing a LacP.E. expression strain, inducing expression and purifying. High per...

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Hauptverfasser: HAO WENBO, JIA MENGSHUANG, ZHANG WEI, GU XIAODAN, YU XIAOHU
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention relates to bacterial laccase LacP.E., a coding gene and application thereof, and a feed additive, and belongs to the technical field of enzyme engineering. The bacterial laccase LacP.E. is obtained by constructing a LacP.E. expression strain, inducing expression and purifying. High performance liquid chromatography detection and analysis show that the laccase LacP.E. can degrade aflatoxin, zearalenone, vomitoxin, ochratoxin, T-2 toxin and fumonisins at the same time without depending on any mediator, and can be widely applied to the field of detoxification of mycotoxin in feed. 本发明涉及细菌漆酶Lac_P.E.、编码基因及其应用和饲料添加剂,属于酶工程技术领域。本发明通过构建Lac_P.E.表达菌株,诱导表达、纯化后获得细菌漆酶Lac_P.E.。通过高效液相色谱检测分析发现,漆酶Lac_P.E.能够不依赖任何介体同时降解黄曲霉毒素、玉米赤霉烯酮、呕吐毒素、赭曲霉毒素、T-2毒素和伏马毒素,可广泛用于饲料霉菌毒素脱毒领域。