Method for producing beta-glucosidase by utilizing chaetomium globosum DX-THS3 and application

The invention belongs to the technical field of fungal fermentation, and provides a method for producing beta-glucosidase by utilizing chaetomium globosum DX-THS3 and application of the beta-glucosidase. The form of the chaetomium globosum DX-THS3 is controlled by adding different nitrogen sources,...

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Hauptverfasser: TAKANAMI RYO, ZHU DU, WANG YA, XIAO YIWEN, HUANG WANFENG, WU WENTING, LIANG WEIZHONG
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention belongs to the technical field of fungal fermentation, and provides a method for producing beta-glucosidase by utilizing chaetomium globosum DX-THS3 and application of the beta-glucosidase. The form of the chaetomium globosum DX-THS3 is controlled by adding different nitrogen sources, and the beta-glucosidase yield of the finally obtained chaetomium globosum DX-THS3 is 3.5 times of that of a non-optimized nitrogen source. The diameter of a small sphere of the chaetomium globosum DX-THS3 under the condition of 7g/L peptone is 1.5 +/-0.65 mm, and isoliquiritin in liquorice can be hydrolyzed and converted into isoliquiritigenin. Besides, a 5L bioreactor is adopted for expression of the beta-glucosidase, the activity of the beta-glucosidase can reach 680U/mL under the optimal fermentation condition, and a strategy is provided for designing the form of filamentous fungi by utilizing a nitrogen source during industrial production of the isoliquiritigenin. 本发明属于真菌发酵技术领域,本发明提供了一种利用球毛壳菌DX-THS3生产β-葡萄糖苷酶的方