Selective culture medium of actinobacillus pleuropneumoniae as well as preparation method and application of selective culture medium

The invention relates to the technical field of isolated culture of microorganisms, in particular to a selective culture medium of actinobacillus pleuropneumoniae as well as a preparation method and application of the selective culture medium. Wherein the component A is prepared from peptone, yeast extract, NaCl, crystal violet, 3 # bile salt, nicotine adenine dinucleotide, agar, nystatin and lincomycin, and the pH value is adjusted to 7.3 +/-0.1 with sodium hydroxide; adding distilled water until the volume is 900mL; and the component B is 95 to 100 mL of fetal calf serum. Compared with the prior art, the separation medium has the advantages that the separation rate is doubled, the separation time is shortened by more than two times, meanwhile, the biological characteristics of the separated APP strain are not influenced, the separation medium can be used for rapid separation and identification of the strain, the separation and identification cost is reduced, and the separation accuracy is improved. 本发明涉及微生物