Preparation and application of anti-human CDK4 recombinant antibody

The invention relates to the technical field of antibodies, in particular to preparation and application of an anti-human CDK4 recombinant antibody. According to the CDK4 recombinant antibody provided by the invention, the recombinant antibody is prepared by constructing a human CDK4 antibody expression vector and adopting a CHO expression system, and meanwhile, the specificity verification of the antibody is carried out through a western blot method and an immunohistochemical method, so that the recombinant antibody provided by the invention is proved to be strong in specificity and stable in antibody property, and meets the repeatability of an experiment; the method is suitable for industrial production and application. 本发明涉及抗体技术领域，具体涉及抗人CDK4重组抗体的制备及应用。本发明提供的CDK4重组抗体，通过构建人源CDK4抗体表达载体，并采用CHO表达系统进行重组抗体的制备，同时通过蛋白印迹法及免疫组织化学法进行抗体的特异性验证，证明本发明提供的重组抗体特异性强，抗体性质稳定，满足了实验的可重复性，适用于工业化生产及应用。