Limited hole heat cycle device

A limited pore thermal cycle device for sample preparation and real-time fluorescence detection is described. The limited aperture thermal cycle device includes a heating block having a sample aperture and at least one reaction aperture, and an optical block including respective means for measuring real-time fluorescence in each reaction aperture. The limited pore thermal cycle device includes means for efficient heating and cooling of the reaction and sample pores for real-time fluorescence detection. The structure of the heating block provides means for efficient heating and cooling by raising each of the sample well and the at least one reaction well above the heating block base such that the sample well and the at least one reaction well are not surrounded by the metal heating block. 说明了一种用于样品制备和实时荧光检测的有限孔热循环装置。该有限孔热循环装置包括具有样品孔和至少一个反应孔的加热块以及包括用于测量各反应孔中的实时荧光的相应装置的光学块。该有限孔热循环装置包括用于反应孔和样品孔的有效加热和冷却以进行实时荧光检测的装置。加热块的结构提供了用于有效加热和冷却的装置，通过使样品孔和至少一个反应孔中的各者上升到加热块底座上方，使得样品孔和至少一个反应孔不被金属加热块包围。