Enzyme linked kit for detecting methimazole drug in livestock and poultry products

The invention relates to an enzyme linked kit for detecting methimazole medicine in livestock and poultry products. Comprising a microporous reaction plate coated with a methimazole coupled protein antigen, a plurality of methimazole standard substance solutions with different concentrations, an antibody working solution added with a murine anti-methimazole antibody, an enzyme marker added with a horseradish peroxidase labeled anti-mouse IgG antibody, a color developing agent containing TMB, a stop solution, a washing solution and a sample diluent. An indirect competitive ELISA method is adopted, the sensitivity is high, the specificity is high, and the methimazole drug residues in a product can be rapidly and accurately detected. 本发明涉及一种检测畜禽产品中甲巯咪唑药物的酶联试剂盒，包括包被有甲巯咪唑偶联蛋白抗原的微孔反应板、多份浓度不同的甲巯咪唑标准品溶液、添加有鼠源抗甲巯咪唑抗体的抗体工作液、添加有辣根过氧化酶标记的抗小鼠IgG抗体的酶标记物、含有TMB的显色剂、终止液、洗涤液、样本稀释液。该发明采用间接竞争ELISA方法，具有灵敏度高、特异性强、能快速准确的检测产品中甲巯咪唑药物的残留。