Construction method and application of chicken's gizzard membrane UPLC characteristic spectrum

The invention relates to a construction method of an endothelium corneum gigeriae galli UPLC specific chromatogram, which comprises the following steps: preparing a reference substance solution, namely preparing an endothelium corneum gigeriae galli reference substance solution and preparing a reference substance solution of a reference substance, the reference substance comprises indole-3-formaldehyde, genistin, daidzein, daidzein and genistein; preparing a test solution of an endothelium corneum gigeriae galli sample, wherein the endothelium corneum gigeriae galli sample is an endothelium corneum gigeriae galli And detecting the reference solution and the test solution of the endothelium corneum gigeriae galli sample through ultra-high performance liquid chromatography. The method can be used for distinguishing and identifying the endothelium corneum gigeriae galli and other different parts of domestic chicken or other poultry counterfeit products. 本申请涉及一种鸡内金UPLC特征图谱的构建方法，包括如下步骤：制备参照物溶液，包括制备鸡内金对照药材参照物溶液以及制备