Method for preparing high-purity and high-stability protein

According to the method, medium-chain and long-chain fatty acid ligands are added, and charge heterogeneity protein is removed through anion or/and cation chromatography, so that the high-purity and high-stability recombinant human albumin is obtained. The method is characterized in that the mixed solution is a mixed solution of a fatty acid mixture and poloxamer; the molar ratio of oleic acid to myristic acid to sodium palmitate to sodium stearate to recombinant human albumin is 0.3: 0.3: 0.3: 0.5: 1; truncated albumin and mismatched or modified albumin in recombinant albumin can be effectively removed through sequential use of ion exchange chromatography, so that charge heterogeneity removed albumin and modified albumin are obtained, and a high-purity and high-stability human albumin sample is obtained. 本发明为采用添加中长链脂肪酸配体，通过阴离子或/和阳离子层析去除电荷异质性蛋白，获得高纯度高稳定性重组人白蛋白的方法。包含如下步骤：本发明的独特之处在于所述的混合液为脂肪酸混合物和泊洛沙姆的混合液；油酸、肉豆蔻酸、棕榈酸钠、硬脂酸钠和重组人白蛋白的摩尔比为0.3：0.3：0.3：0.5：1；序贯使用离子交换层析可以有效地去除重组白蛋白中的截断的白蛋白、错配或修饰的白蛋白，从而得到去除电荷异质性白蛋白、修饰后的白