Hexavalent norovirus VLPs vaccine and preparation method thereof
The invention discloses a sexavalent norovirus VLPs vaccine preparation and a preparation method of the sexavalent norovirus VLPs vaccine preparation. The sexavalent norovirus VLPs vaccine preparation contains six proteins as shown in SEQ ID No.2, SEQ ID No.4, SEQ ID No.6, SEQ ID No.8, SEQ ID No.10...
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| creator | XING MAN ZHOU DONGMING XIE DI LYU LIHUI REN JILING LIU HUI HOU WENLI |
| description | The invention discloses a sexavalent norovirus VLPs vaccine preparation and a preparation method of the sexavalent norovirus VLPs vaccine preparation. The sexavalent norovirus VLPs vaccine preparation contains six proteins as shown in SEQ ID No.2, SEQ ID No.4, SEQ ID No.6, SEQ ID No.8, SEQ ID No.10 and SEQ ID No.12. A pichia pastoris expression system is adopted, the six-order norola vaccine is researched and developed, through sequencing, enzyme digestion, exogenous gene expression identification, high expression strain screening, sucrose density centrifugation and VLP electron microscope identification, the purity of the six-order candidate vaccine VLP can reach 95% or above, each VLP particle is complete and uniform in size, and it is indicated that the obtained VLP is complete in structure and stable in performance. After mice are immunized by the six-order VLP vaccine, a lasting and high-titer antibody reaction can be induced, and a specific blocking antibody and T cell immune reaction can be generated. |
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The sexavalent norovirus VLPs vaccine preparation contains six proteins as shown in SEQ ID No.2, SEQ ID No.4, SEQ ID No.6, SEQ ID No.8, SEQ ID No.10 and SEQ ID No.12. A pichia pastoris expression system is adopted, the six-order norola vaccine is researched and developed, through sequencing, enzyme digestion, exogenous gene expression identification, high expression strain screening, sucrose density centrifugation and VLP electron microscope identification, the purity of the six-order candidate vaccine VLP can reach 95% or above, each VLP particle is complete and uniform in size, and it is indicated that the obtained VLP is complete in structure and stable in performance. After mice are immunized by the six-order VLP vaccine, a lasting and high-titer antibody reaction can be induced, and a specific blocking antibody and T cell immune reaction can be generated.</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; HUMAN NECESSITIES ; HYGIENE ; MEDICAL OR VETERINARY SCIENCE ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; ORGANIC CHEMISTRY ; PEPTIDES ; PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2023</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20230203&DB=EPODOC&CC=CN&NR=115677838A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25543,76294</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20230203&DB=EPODOC&CC=CN&NR=115677838A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>XING MAN</creatorcontrib><creatorcontrib>ZHOU DONGMING</creatorcontrib><creatorcontrib>XIE DI</creatorcontrib><creatorcontrib>LYU LIHUI</creatorcontrib><creatorcontrib>REN JILING</creatorcontrib><creatorcontrib>LIU HUI</creatorcontrib><creatorcontrib>HOU WENLI</creatorcontrib><title>Hexavalent norovirus VLPs vaccine and preparation method thereof</title><description>The invention discloses a sexavalent norovirus VLPs vaccine preparation and a preparation method of the sexavalent norovirus VLPs vaccine preparation. The sexavalent norovirus VLPs vaccine preparation contains six proteins as shown in SEQ ID No.2, SEQ ID No.4, SEQ ID No.6, SEQ ID No.8, SEQ ID No.10 and SEQ ID No.12. A pichia pastoris expression system is adopted, the six-order norola vaccine is researched and developed, through sequencing, enzyme digestion, exogenous gene expression identification, high expression strain screening, sucrose density centrifugation and VLP electron microscope identification, the purity of the six-order candidate vaccine VLP can reach 95% or above, each VLP particle is complete and uniform in size, and it is indicated that the obtained VLP is complete in structure and stable in performance. 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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS SPIRITS VINEGAR WINE |
| title | Hexavalent norovirus VLPs vaccine and preparation method thereof |
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