Hexavalent norovirus VLPs vaccine and preparation method thereof

The invention discloses a sexavalent norovirus VLPs vaccine preparation and a preparation method of the sexavalent norovirus VLPs vaccine preparation. The sexavalent norovirus VLPs vaccine preparation contains six proteins as shown in SEQ ID No.2, SEQ ID No.4, SEQ ID No.6, SEQ ID No.8, SEQ ID No.10 and SEQ ID No.12. A pichia pastoris expression system is adopted, the six-order norola vaccine is researched and developed, through sequencing, enzyme digestion, exogenous gene expression identification, high expression strain screening, sucrose density centrifugation and VLP electron microscope identification, the purity of the six-order candidate vaccine VLP can reach 95% or above, each VLP particle is complete and uniform in size, and it is indicated that the obtained VLP is complete in structure and stable in performance. After mice are immunized by the six-order VLP vaccine, a lasting and high-titer antibody reaction can be induced, and a specific blocking antibody and T cell immune reaction can be generated.