Momordica grosvenori seedling stem tip micro-grafting culture method
The invention discloses a siraitia grosvenorii seedling stem tip micro-grafting culture method which comprises the following steps: taking a non-toxic siraitia grosvenorii tissue culture seedling stem section as a stock, taking a stem tip of a virus-carrying tissue culture seedling which is equal to...
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| creator | LIU YANXUE MO XUEYAN HUANG XUEMEI LIU YUE ZHANG YUHUA ZHOU YIJING LI YUANYUAN HUANG RONGMING |
| description | The invention discloses a siraitia grosvenorii seedling stem tip micro-grafting culture method which comprises the following steps: taking a non-toxic siraitia grosvenorii tissue culture seedling stem section as a stock, taking a stem tip of a virus-carrying tissue culture seedling which is equal to or less than 0.5 mm as a scion, grafting to the stock, and culturing to obtain a seedling. According to the method, the siraitia grosvenorii in-vitro stem tips equal to or smaller than 0.5 mm are grafted to the non-toxic tissue culture seedling stock at the tissue culture stage to be cultured and grow, a stem tip micro-grafting culture technology is established, and the time for obtaining the regenerated detoxified seedlings is greatly shortened while the detoxification efficiency of stem tip culture is ensured.
本发明公开了罗汉果种苗茎尖微嫁接培养方法，以无毒罗汉果组培苗茎段作为砧木，以带毒组培苗等于小于0.5mm的茎尖为接穗，嫁接于所述砧木，培养至成幼苗。本发明通过把罗汉果等于小于0.5毫米离体茎尖在组培阶段嫁接到无毒组培苗砧木上培养生长，建立了茎尖微嫁接培养技术，在确保茎尖培养脱毒效率的同时，大大缩短了获得再生脱毒种苗的时间。 |
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本发明公开了罗汉果种苗茎尖微嫁接培养方法，以无毒罗汉果组培苗茎段作为砧木，以带毒组培苗等于小于0.5mm的茎尖为接穗，嫁接于所述砧木，培养至成幼苗。本发明通过把罗汉果等于小于0.5毫米离体茎尖在组培阶段嫁接到无毒组培苗砧木上培养生长，建立了茎尖微嫁接培养技术，在确保茎尖培养脱毒效率的同时，大大缩短了获得再生脱毒种苗的时间。</description><language>chi ; eng</language><subject>AGRICULTURE ; ANIMAL HUSBANDRY ; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPSOR SEAWEED ; FISHING ; FORESTRY ; HORTICULTURE ; HUMAN NECESSITIES ; HUNTING ; TRAPPING ; WATERING</subject><creationdate>2023</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20230203&DB=EPODOC&CC=CN&NR=115669389A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25564,76547</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20230203&DB=EPODOC&CC=CN&NR=115669389A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>LIU YANXUE</creatorcontrib><creatorcontrib>MO XUEYAN</creatorcontrib><creatorcontrib>HUANG XUEMEI</creatorcontrib><creatorcontrib>LIU YUE</creatorcontrib><creatorcontrib>ZHANG YUHUA</creatorcontrib><creatorcontrib>ZHOU YIJING</creatorcontrib><creatorcontrib>LI YUANYUAN</creatorcontrib><creatorcontrib>HUANG RONGMING</creatorcontrib><title>Momordica grosvenori seedling stem tip micro-grafting culture method</title><description>The invention discloses a siraitia grosvenorii seedling stem tip micro-grafting culture method which comprises the following steps: taking a non-toxic siraitia grosvenorii tissue culture seedling stem section as a stock, taking a stem tip of a virus-carrying tissue culture seedling which is equal to or less than 0.5 mm as a scion, grafting to the stock, and culturing to obtain a seedling. According to the method, the siraitia grosvenorii in-vitro stem tips equal to or smaller than 0.5 mm are grafted to the non-toxic tissue culture seedling stock at the tissue culture stage to be cultured and grow, a stem tip micro-grafting culture technology is established, and the time for obtaining the regenerated detoxified seedlings is greatly shortened while the detoxification efficiency of stem tip culture is ensured.
本发明公开了罗汉果种苗茎尖微嫁接培养方法，以无毒罗汉果组培苗茎段作为砧木，以带毒组培苗等于小于0.5mm的茎尖为接穗，嫁接于所述砧木，培养至成幼苗。本发明通过把罗汉果等于小于0.5毫米离体茎尖在组培阶段嫁接到无毒组培苗砧木上培养生长，建立了茎尖微嫁接培养技术，在确保茎尖培养脱毒效率的同时，大大缩短了获得再生脱毒种苗的时间。</description><subject>AGRICULTURE</subject><subject>ANIMAL HUSBANDRY</subject><subject>CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPSOR SEAWEED</subject><subject>FISHING</subject><subject>FORESTRY</subject><subject>HORTICULTURE</subject><subject>HUMAN NECESSITIES</subject><subject>HUNTING</subject><subject>TRAPPING</subject><subject>WATERING</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2023</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZHDxzc_NL0rJTE5USC_KLy5LzcsvylQoTk1NycnMS1coLknNVSjJLFDIzUwuytdNL0pMKwGJJ5fmlJQWpSrkppZk5KfwMLCmJeYUp_JCaW4GRTfXEGcP3dSC_PjU4oLE5NS81JJ4Zz9DQ1MzM0tjC0tHY2LUAAADQjOD</recordid><startdate>20230203</startdate><enddate>20230203</enddate><creator>LIU YANXUE</creator><creator>MO XUEYAN</creator><creator>HUANG XUEMEI</creator><creator>LIU YUE</creator><creator>ZHANG YUHUA</creator><creator>ZHOU YIJING</creator><creator>LI YUANYUAN</creator><creator>HUANG RONGMING</creator><scope>EVB</scope></search><sort><creationdate>20230203</creationdate><title>Momordica grosvenori seedling stem tip micro-grafting culture method</title><author>LIU YANXUE ; MO XUEYAN ; HUANG XUEMEI ; LIU YUE ; ZHANG YUHUA ; ZHOU YIJING ; LI YUANYUAN ; HUANG RONGMING</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN115669389A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2023</creationdate><topic>AGRICULTURE</topic><topic>ANIMAL HUSBANDRY</topic><topic>CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPSOR SEAWEED</topic><topic>FISHING</topic><topic>FORESTRY</topic><topic>HORTICULTURE</topic><topic>HUMAN NECESSITIES</topic><topic>HUNTING</topic><topic>TRAPPING</topic><topic>WATERING</topic><toplevel>online_resources</toplevel><creatorcontrib>LIU YANXUE</creatorcontrib><creatorcontrib>MO XUEYAN</creatorcontrib><creatorcontrib>HUANG XUEMEI</creatorcontrib><creatorcontrib>LIU YUE</creatorcontrib><creatorcontrib>ZHANG YUHUA</creatorcontrib><creatorcontrib>ZHOU YIJING</creatorcontrib><creatorcontrib>LI YUANYUAN</creatorcontrib><creatorcontrib>HUANG RONGMING</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>LIU YANXUE</au><au>MO XUEYAN</au><au>HUANG XUEMEI</au><au>LIU YUE</au><au>ZHANG YUHUA</au><au>ZHOU YIJING</au><au>LI YUANYUAN</au><au>HUANG RONGMING</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Momordica grosvenori seedling stem tip micro-grafting culture method</title><date>2023-02-03</date><risdate>2023</risdate><abstract>The invention discloses a siraitia grosvenorii seedling stem tip micro-grafting culture method which comprises the following steps: taking a non-toxic siraitia grosvenorii tissue culture seedling stem section as a stock, taking a stem tip of a virus-carrying tissue culture seedling which is equal to or less than 0.5 mm as a scion, grafting to the stock, and culturing to obtain a seedling. According to the method, the siraitia grosvenorii in-vitro stem tips equal to or smaller than 0.5 mm are grafted to the non-toxic tissue culture seedling stock at the tissue culture stage to be cultured and grow, a stem tip micro-grafting culture technology is established, and the time for obtaining the regenerated detoxified seedlings is greatly shortened while the detoxification efficiency of stem tip culture is ensured.
本发明公开了罗汉果种苗茎尖微嫁接培养方法，以无毒罗汉果组培苗茎段作为砧木，以带毒组培苗等于小于0.5mm的茎尖为接穗，嫁接于所述砧木，培养至成幼苗。本发明通过把罗汉果等于小于0.5毫米离体茎尖在组培阶段嫁接到无毒组培苗砧木上培养生长，建立了茎尖微嫁接培养技术，在确保茎尖培养脱毒效率的同时，大大缩短了获得再生脱毒种苗的时间。</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | AGRICULTURE ANIMAL HUSBANDRY CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPSOR SEAWEED FISHING FORESTRY HORTICULTURE HUMAN NECESSITIES HUNTING TRAPPING WATERING |
| title | Momordica grosvenori seedling stem tip micro-grafting culture method |
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