Momordica grosvenori seedling stem tip micro-grafting culture method

The invention discloses a siraitia grosvenorii seedling stem tip micro-grafting culture method which comprises the following steps: taking a non-toxic siraitia grosvenorii tissue culture seedling stem section as a stock, taking a stem tip of a virus-carrying tissue culture seedling which is equal to or less than 0.5 mm as a scion, grafting to the stock, and culturing to obtain a seedling. According to the method, the siraitia grosvenorii in-vitro stem tips equal to or smaller than 0.5 mm are grafted to the non-toxic tissue culture seedling stock at the tissue culture stage to be cultured and grow, a stem tip micro-grafting culture technology is established, and the time for obtaining the regenerated detoxified seedlings is greatly shortened while the detoxification efficiency of stem tip culture is ensured. 本发明公开了罗汉果种苗茎尖微嫁接培养方法，以无毒罗汉果组培苗茎段作为砧木，以带毒组培苗等于小于0.5mm的茎尖为接穗，嫁接于所述砧木，培养至成幼苗。本发明通过把罗汉果等于小于0.5毫米离体茎尖在组培阶段嫁接到无毒组培苗砧木上培养生长，建立了茎尖微嫁接培养技术，在确保茎尖培养脱毒效率的同时，大大缩短了获得再生脱毒种苗的时间。