Method for producing target cell, method for producing product using target cell, and serum-free medium

The present invention relates to a method for producing a cell of interest, comprising culturing a starting material cell with a serum-free medium comprising: a culture factor which is at least one type selected from the group consisting of a growth factor and a differentiation-inducing factor; huma...

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Hauptverfasser: MOGI ITSURO, MINAMI ITSUNARI, ETO KOJI
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MINAMI ITSUNARI
ETO KOJI
description The present invention relates to a method for producing a cell of interest, comprising culturing a starting material cell with a serum-free medium comprising: a culture factor which is at least one type selected from the group consisting of a growth factor and a differentiation-inducing factor; human serum albumin; and an additive which is at least one selected from the group consisting of an Src inhibitor, a PKC activator, methylcellulose, an Essental 8 culture medium, recombinant human serum albumin and serum albumin of animals other than human. 本发明涉及目的细胞的生产方法,其包含用无血清培养基培养原料细胞以生产目的细胞,所述无血清培养基包含下述成分:培养因子,其为选自生长因子和分化诱导因子中的至少一种;人血清白蛋白;和添加物,其为选自Src抑制剂、PKC激活剂、甲基纤维素、Essential 8培养基、重组人血清白蛋白和人以外的动物血清白蛋白中的至少一种。
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subjects BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
title Method for producing target cell, method for producing product using target cell, and serum-free medium
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