Method for producing target cell, method for producing product using target cell, and serum-free medium

The present invention relates to a method for producing a cell of interest, comprising culturing a starting material cell with a serum-free medium comprising: a culture factor which is at least one type selected from the group consisting of a growth factor and a differentiation-inducing factor; huma...

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Hauptverfasser: MOGI ITSURO, MINAMI ITSUNARI, ETO KOJI
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The present invention relates to a method for producing a cell of interest, comprising culturing a starting material cell with a serum-free medium comprising: a culture factor which is at least one type selected from the group consisting of a growth factor and a differentiation-inducing factor; human serum albumin; and an additive which is at least one selected from the group consisting of an Src inhibitor, a PKC activator, methylcellulose, an Essental 8 culture medium, recombinant human serum albumin and serum albumin of animals other than human. 本发明涉及目的细胞的生产方法,其包含用无血清培养基培养原料细胞以生产目的细胞,所述无血清培养基包含下述成分:培养因子,其为选自生长因子和分化诱导因子中的至少一种;人血清白蛋白;和添加物,其为选自Src抑制剂、PKC激活剂、甲基纤维素、Essential 8培养基、重组人血清白蛋白和人以外的动物血清白蛋白中的至少一种。